Protein based hydrogel research group


Dr. Bae Hoon Lee
Principal Investigator
Wenzhou institute, UCAS
Tel: +86-577-88017564
Email: bhlee@wiucas.ac.cn

Dr. Lee has nearly 20 years of research and development experience in the field of biomedical gels at various places (China, US, Singapore, and Korea). In recent years, he has performed a lot of basic research work in the field of protein-based hydrogels and obtained a series of scientific research results. The results have laid a solid foundation for the further industrialization of protein-based hydrogels in the field of bio-ink and 3D bio-printing. In addition, he also has used protein-based hydrogels to construct highly ordered and connected porous three-dimensional cell scaffolds with full protein bases, which has a very prominent application development prospect in the field of three-dimensional cell culture and drug screening.

Our team consists of researchers with various backgrounds (chemistry, materials science, biology, and engineering). Our team focuses on the development of protein-based hydrogels for bioapplications including tissue engineering, drug screening, and 3D bioprinting. Protein-based materials exhibit excellent biocompatibility and biological activities. However, their poor mechanical properties limit their wide ranges of bioapplications. Here, we have functionalized protein materials via methacryloylation so that they can be solidified upon exposure to light. Photocrosslinking imparts the spatiotemporal control over biofabrication as well as tailorable mechanical properties. Photocuring is conducted under a mild condition in the presence of a photoinitiator, relatively leading to good biocompatibility. Photocurable protein-based hydrogels have been used to fabricate highly ordered 3D scaffolds/tissue analogues for 3D culture and drug screening applications. Besides, they have been used to print various tissue constructs. Photocurable protein-based hydrogels could become versatile materials for various bioapplications.

Research Interests
photocurable protein-based hydrogels; bioinks for 3D bioprinting; highly ordered protein-based scaffolds for drug screening applications; engineered organoids
1. Preparation of various photocurable protein-based hydrogels

2. Biofabrication of 3D tissue constructs with well-defined architecture.

3. 3D printing of protein-based hydrogels with improved printability

Research Achievements
Photocurable bioinks products, including gelatin methacryloyl(GelMA),Albumin methacryloyl (AlbMA), hyaluronic methacrylate(HAMA),Pluronic diacrylate(Pluronic DA), poly(ethylene glycol diacrylate)(PEGDA),are general materials for biological application, including 3D cell culture, 3D printing, tissue engineering and tissue regeneration

Recent Papers
1. BH Lee+, H Shirahama+, NJ Cho*, LP Tan* (+first authors) “Efficient and controllable synthesis of highly substituted gelatin-methacrylamide for mechanically stiff hydrogels” RSC Adv., 2015, 5, 106094-106097.
2. H Shirahama+, BH Lee+, LP Tan*, NJ Cho* (+first authors) “Precise Tuning of Facile One-pot Gelatin Methacryloyl (GelMA) Hydrogel Synthesis” Scientific Report, 2016, 6, 31036
3. BH Lee+*, N Lum+, LY Seow+, PQ Lim, LP Tan* “Synthesis and Characterization of Types A and B Gelatin Methacryloyl for Bioink Applications” Materials, 2016, 9(10), 797
4. BH Lee+, H Shirahama+, MH Kim+, JH Lee, LP Tan*, NJ Cho* “Colloidal Templating of Highly Ordered Gelatin Methacryloyl-Based Hydrogel Platforms for Three-Dimensional Tissue Analogues” NPG Asia Materials, 2017, 9, e412
5. M Zhou+, BH Lee+, LP Tan* (+first authors) “A dual crosslinking strategy to tailor rheological properties of gelatin methacryloyl” International Journal of Bioprinting, 2017, 3(2), 130
6. WS Lim+, K Chen+, TW Chong, GM Xiong, W Birch, J Pan, BH Lee, PS Er, AV Salvekar, S Venkatraman*, Y Huang* “A bilayer swellable drug-eluting ureteric stent: Localized drug delivery to treat urothelial diseases” Biomaterials, 2018, 165, 25-38
7. J Zheng, M Zhu, G Ferracci, N-J Cho*, BH Lee* “Hydrolytic stability of methacrylamide and methacrylate in gelatin methacryloyl and decoupling of gelatin methacrylamide from GelMA through hydrolysis” Macromol Chem Phys, 2018, 219, 1800266
8. F Kong, BH Lee*, K Wei* (*corresponding authors) “5-Hydroxymethylfurfural mitigates lipopolysaccharide-stimulated inflammation via suppression of MAPK, NF-κB, and mTOR activation in RAW 264.7 cells” Molecules, 2019, 24, 275
9. M Zhou, BH Lee*, YJ Tan, LP Tan* (*corresponding authors) “Microbial Transglutaminase Induced Controlled Crosslinking of Gelatin Methacryloyl to Tailor Rheological Properties for 3D Printing” Biofabrication, 2019, 11:025011 DOI: 10.1088/1758-5090/ab063f
10. MengXiang Zhu, Yingying Wang, Gaia Ferracci, Jing Zheng, Nam-Joon Cho*, Bae Hoon Lee* (*corresponding authors) “Gelatin methacryloyl with a high degree of controllability and batch-to-batch consistency for hydrogel fabrication” Scientific Reports, 2019 9:6863
11. Fanhui Kong+, Conghui Fan, Yulian Yang, Bae Hoon Lee*+, and Kun Wei * (*corresponding and +first authors) “5-hydroxymethylfurfural-embedded poly (vinyl alcohol)/sodium alginate hybrid hydrogels accelerate wound healing” International Journal of Biological Macromolecules, 2019, 138, 933 DOI: 10.1016/j.ijbiomac.2019.07.152
12. Dattatrya Shetti, Bao Zhang, Conghui Fan, Canlong Mo, Bae Hoon Lee*, and Kun Wei* (*corresponding authors) “Low dose of Paclitaxel Combined with XAV939 Attenuates Metastasis, Angiogenesis, and Growth in Breast Cancer by Suppressing Wnt Signaling” cells (MDPI), 2019, 8, 892 DOI: 10.3390/cells8080892

Patents
一种用于细胞和组织培养的高取代白蛋白甲基丙烯酰基水凝胶的制备方法(201911170929. 5)_2019年11月26日

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